ABSTRACT
Objective: To evaluate the effect of different stem branches of Bupleurum chinense on saikosaponins content in roots and its root yield. Methods: The contents of saikosaponin a, saikosaponin d, and total saikosaponins in the roots of B. chinense were determined by HPLC and UV-vis spectrophotometry. And parameters of growth characteristics were analyzed. Results: The contents of saikosaponin a, saikosaponin d, and total saikosaponins in the roots of B. chinense and its root yield were the highest when the B. chinense stem had no branch or two branches. And with the increase of stem branch's numbers, the content of saikosaponins in the roots of B. chinense and its root yield decreased. Conclusion: Different stem branches have a significant effect on the content of saikosaponins in the roots of B. chinense and its root yield.